Quality of Life (Banja Luka) 9(1-2): 5-12


Chemical Composition and Antibacterial Activity of the Essential Oil of Menthae piperitae L.
Željka Marjanović-Balaban1, Ljiljana Stanojević 2, Vesna Kalaba3, Jelena Stanojević2, Dragan Cvetković2, Milorad Cakić2, Vesna Gojković4
1 University of Banja Luka, Faculty of Forestry, Republic of Srpska, Bosnia and Herzegovina
2 University of Niš, Faculty of Technology, Bulevar Oslobođenja 124, 16000 Leskovac, Serbia
3 Veterinary Institute of the Republic of Srpska "Dr Vaso Butozan" Banja Luka, Republic of Srpska, Bosnia and Herzegovina
4 University of East Sarajevo – Zvornik, Faculty of Technology, Republic of Srpska, Bosnia and Herzegovina


Abstract: The subject of this study was the examination of the chemical composition and antimicrobial activity of peppermint oil (Menthae piperitae L.), that was produced from the plant collected from the plantation in the city of Banja Luka. To examine the chemical composition of the Mentha piperita essential oil we used the GC-FID method. On it's chromatogram it was clearly shown that the main components of the oil were menthole with the content of 43.66%, menthone 20.02%, iso-menthone 7.73%, following by 1,8-cineole with a percentage of 6.49% and menthil-acetate with 3.31%. The content of the previously mentioned components were all in accordance with the suggestions made by Ph.Eur 8.0. For the purpose of examination antimicrobial activity of the peppermint oil we used agar diffusion method. The results have shown that etheric oil of Menthae piperitae reflects very good antimicrobial activity on all bacterial cultures that we used in this study. The inhibiton zones moved from 12 mm for Pseudomonas aeruginosa to 37.66 mm for Staphylococcus aureus. Such an outcome of our study, and many others from all around the world, indicates the future potential use of peppermint oil in the treatment of bacterial infections, maybe even lowering the use of antibiotics.

Key words:  Essential oil, Menthae piperitae L., GC-MS analysis, Antimicrobial activity.

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